C3 Goat Polyclonal Antibody – AP21439FC-N (2024)

FAQs

What is a polyclonal antibody response? ›

Polyclonal antibodies (PAbs) are a mixture of antibodies that are secreted by different B cell lineages. These antibodies are actually a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope on an antigen.

Polyclonal and monoclonal antibodies to BBTV are used in enzyme-linked immunosorbent assay (ELISA) to detect the virus in field and tissue culture plants and can detect the virus in single viruliferous aphids. PCR was shown to be about a thousand times more sensitive than ELISA or dot blots with DNA probes.

Its use results in rapid T-cell depletion, which can result in a cytokine release syndrome characterized by fever, diarrhea, nausea, vomiting, headaches, myalgias, and/or shortness of breath.

Polyclonal antibodies work in the following ways: They target various immune system proteins including lymphocyte surface proteins, granulocytes, platelets, bone marrow cells, and other cell types. They suppress the immune system to prevent renal transplant rejection.

Although the polyclonal response confers advantages on the immune system, in particular, greater probability of reacting against pathogens, it also increases chances of developing certain autoimmune diseases resulting from the reaction of the immune system against native molecules produced within the host.

: produced by, involving, or being cells derived from two or more cells of different ancestry or genetic constitution.

Disadvantages of using polyclonal antibodies: They are prone to batch variability (i.e. the antibody population produced against the same antigen in the same animal may not be exactly the same).

What is polyclonal antibody production? Polyclonal antibodies (pAbs) are produced by injecting a specific antigen into lab animals, such as rabbits and goats, etc. The animal is immunized repeatedly to obtain higher titers of antibodies specific for the antigen.

Because monoclonal antibodies specifically detect a particular epitope on the antigen, they are less likely than polyclonal antibodies to cross-react with other proteins.

Conversely, polyclonal activation can be triggered by microorganisms to avoid the host-specific, immune response by activating B cell clones, which produce nonmicroorganism-specific antibodies.

What causes polyclonal increase? ›

Liver disease, autoimmune disease, chronic viral or bacterial infections and various malignancies may cause a polyclonal rise in the gamma fraction (see Table 2 below). Polyclonal pattern serum protein electrophoresis (SPEP).

What are examples of polyclonal antibodies? Some examples of polyclonal antibodies include certain cancer treatments that can target various tumor cells. Like immunoassays such as ELISA which are frequently used in research, and immunotherapies among others.

mAbs stem from a single cell clone and exhibit remarkable specificity by binding to a lone epitope on the target antigen with consistent structural uniformity. In contrast, pAbs arise from multiple clones of B cells and provide a more extensive epitope recognition range, resulting in structural diversity.

Polyclonal antibodies are made using several different immune cells. They will have the affinity for the same antigen but different epitopes, while monoclonal antibodies are made using identical immune cells that are all clones of a specific parent cell.

In contrast to polyclonal antibodies, which are produced by multiple immune cells, monoclonal antibodies are generated by identical immune cells which are clones of a single parent cell. This means that the antibody recognizes only a single epitope of an antigen and is extremely specific.