C3 Goat Polyclonal Antibody – AP21439FC-N (2024)

CAT#: AP21439FC-N

    C3 goat polyclonal antibody, FITC

    Conjugation: Biotin FITC HRP

    USD 540.00

    2 Weeks*


      • 1 ml


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    C3 Goat Polyclonal Antibody – AP21439FC-N (1)

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    Product Data
    Applications ID, IF, IHC, IP
    Recommended Dilution This Fluorescent immunoconjugate antibody to Rat C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain
    complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings.
    Recommended Dilutions: 1/20-1/80.
    Reactivities Rat
    Host Goat
    Isotype IgG
    Clonality Polyclonal
    Immunogen C3c isolated and purified from pooled normal Rat serum.
    Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Specificity In Immunoelectrophoresis against fresh Rat serum, a single precipitin line is obtained in the beta-1 region representing native C3.
    Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain.
    The product does not react with any other protein components of Rat serum or plasma.
    The antiserum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
    Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
    Formulation PBS, pH 7.2 without preservatives and foreign proteins
    Label: FITC
    State: Lyophilized hyperimmune IgG fraction
    Label: Fluorescein Isothiocyanate Isomer 1
    Absorption emission: 492 nm / 515 nm
    Molar radio: Fluorescein/IgG ~1.8
    Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
    Concentration lot specific
    Purification The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion
    Conjugation FITC
    Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
    If a slight precipitation occurs upon storage, this should be removed by centrifugation.
    Stability Shelf life: one year from despatch.
    Gene Name complement component 3
    Database Link
    Background C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways.
    Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.
    Synonyms CPAMD1, Complement component 3
    Note This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
    Reference Data


    Product Manuals
    • Western Blotting Luminol Kit
    • Antibody and Western Standard
    • SDS for AP21439FC-N


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    *Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

    C3 Goat Polyclonal Antibody – AP21439FC-N (2024)


    What is a polyclonal antibody response? ›

    Polyclonal antibodies (PAbs) are a mixture of antibodies that are secreted by different B cell lineages. These antibodies are actually a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope on an antigen.

    What is the significance of polyclonal antibodies? ›

    Polyclonal and monoclonal antibodies to BBTV are used in enzyme-linked immunosorbent assay (ELISA) to detect the virus in field and tissue culture plants and can detect the virus in single viruliferous aphids. PCR was shown to be about a thousand times more sensitive than ELISA or dot blots with DNA probes.

    What are the side effects of polyclonal antibodies? ›

    Its use results in rapid T-cell depletion, which can result in a cytokine release syndrome characterized by fever, diarrhea, nausea, vomiting, headaches, myalgias, and/or shortness of breath.

    What body system does polyclonal antibodies affect? ›

    Polyclonal antibodies work in the following ways: They target various immune system proteins including lymphocyte surface proteins, granulocytes, platelets, bone marrow cells, and other cell types. They suppress the immune system to prevent renal transplant rejection.

    Is polyclonal good or bad? ›

    Although the polyclonal response confers advantages on the immune system, in particular, greater probability of reacting against pathogens, it also increases chances of developing certain autoimmune diseases resulting from the reaction of the immune system against native molecules produced within the host.

    What does polyclonal mean in medical terms? ›

    : produced by, involving, or being cells derived from two or more cells of different ancestry or genetic constitution.

    Which of the following is a downside of polyclonal antibodies? ›

    Disadvantages of using polyclonal antibodies: They are prone to batch variability (i.e. the antibody population produced against the same antigen in the same animal may not be exactly the same).

    Where do polyclonal antibodies come from? ›

    What is polyclonal antibody production? Polyclonal antibodies (pAbs) are produced by injecting a specific antigen into lab animals, such as rabbits and goats, etc. The animal is immunized repeatedly to obtain higher titers of antibodies specific for the antigen.

    What is better polyclonal or monoclonal? ›

    Because monoclonal antibodies specifically detect a particular epitope on the antigen, they are less likely than polyclonal antibodies to cross-react with other proteins.

    What triggers a polyclonal response? ›

    Conversely, polyclonal activation can be triggered by microorganisms to avoid the host-specific, immune response by activating B cell clones, which produce nonmicroorganism-specific antibodies.

    What causes polyclonal increase? ›

    Liver disease, autoimmune disease, chronic viral or bacterial infections and various malignancies may cause a polyclonal rise in the gamma fraction (see Table 2 below). Polyclonal pattern serum protein electrophoresis (SPEP).

    What is an example of a polyclonal antibody? ›

    What are examples of polyclonal antibodies? Some examples of polyclonal antibodies include certain cancer treatments that can target various tumor cells. Like immunoassays such as ELISA which are frequently used in research, and immunotherapies among others.

    What is the difference between polyclonal and monoclonal antibody response? ›

    mAbs stem from a single cell clone and exhibit remarkable specificity by binding to a lone epitope on the target antigen with consistent structural uniformity. In contrast, pAbs arise from multiple clones of B cells and provide a more extensive epitope recognition range, resulting in structural diversity.

    What is the difference between a polyclonal antibody response and a monoclonal antibody response? ›

    Polyclonal antibodies are made using several different immune cells. They will have the affinity for the same antigen but different epitopes, while monoclonal antibodies are made using identical immune cells that are all clones of a specific parent cell.

    What is the difference between monoclonal and polyclonal immune response? ›

    In contrast to polyclonal antibodies, which are produced by multiple immune cells, monoclonal antibodies are generated by identical immune cells which are clones of a single parent cell. This means that the antibody recognizes only a single epitope of an antigen and is extremely specific.

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